RESUMO
A novel Gram-stain-negative, facultatively anaerobic and rod-shaped bacterial strain, designated as DAU312T, was isolated from the sea water of the eastern coast of the Republic of Korea. Optimal growth was observed at 25â°C, pH 7.0-8.0 and with NaCl concentrations of 2.0â% (w/v). Catalase and oxidase activities were detected. On the basis of 16S rRNA gene sequences, strain DAU312T showed the highest similarity (99.2â%) to the type strain Shewanella electrodiphila MAR441T. The complete genome sequence of strain DAU312T contains 4â893â483 bp and 40.5âmol% G+C. Phylogenetic analyses based on 16S rRNA gene sequences and the up-to-date bacterial core genes showed that strain DAU312T, S. electrodiphila MAR441T and S. olleyana were all part of the same monophyletic clade. Their average nucleotide identity, digital DNA-DNA hybridization and two-way average amino acid identity values with each other and type strains of close Shewanella species were 83.4-77.5â%, 27.3-22.0â% and 89.8-81.2â%, respectively. The major cellular fatty acids (>10â%) were iso-C15â:â0, summed feature 3 (C16â:â1 ω7Ñ and/or C16â:â1 ω6Ñ) and C16â:â0. Phosphatidylethanolamine and phosphatidylglycerol were the main polar lipids. The respiratory quinones were Q-7, Q-8, MK-7 and MMK-7. Based on these polyphasic taxonomic findings, the name Shewanella goraebulensis sp. nov. is suggested for strain DAU312T, which is considered to represent a novel species of the genus Shewanella. The type strain is DAU312T (=KCTC 72427 T=JCM 35744T=KCCM 43478T).
